No

STREPPRO LANCEFIELD GROUP A TEST

Effective Date: 
Wed, 03/04/2020
Reviewed: 
Wed, 05/26/2021
Policy: 

Hardy Diagnostics StrepPRO Grouping Kit provides a rapid latex agglutination method for the serological identification of Lancefield group A from isolated colonies of beta-hemolytic Stretococcus species.

Beta-hemolytic streptocicci are the most frequently isolated human pathogens among the representatives of the genus Streptococcus.  Nearly all the beta-hemolytic streptococci possess specific carbohydrate antigens, known as streptococcal grouping antigens.

Hardy Diagnostics StrepPRO Grouping Kit liberates the specific antigen from the bacterial cell wall using a modified nitrous acid extraction.  The extracted antigen, in conjunction with latex agglutination, offers a rapid, sensitive, and specific method for identification of streptococcal group A from primary culture plates.  Extraction Reagents 1 and 2 contain a chemical substance able to extract the streptococcal group specific antigen at room temperature.  Extraction Reagent 3 contains a neutralizing solution.  The neutralized extract can then be identified using blue latex particles sensitized with purified group specific rabbit immunoglobulins.  These blue latex particles agglutinate strongly in the presence of homologous antigen and will not agglutinate when homologous antigen is absent.

MATERIAL SUPPLIED:

  • Blue Latex Suspension Group A
  • Extraction Reagent 1
  • Extraction Reagent 2
  • Extraction Reagent 3
  • Polyvalent Positive Control
  • Latex test Card

MATERIALS REQUIRED BUT NOT SUPPLIED

  • Standard microbiological culture supplies
  • 12X75mm test tubes
  • Timers
  • Applicator sticks

STORAGE AND SHELF LIFE

Upon receipt, store at 2-80 C away from direct light.  Do Not Freeze.  This kit, or any of its reagents, should not be used if there are any signs of discoloration, contamination, or if the expiration date has passed.

When stored as directed, the product may be used and tested up to the expiration date on the product label.

PRECAUTIONS

The Blue Latex Reagents, Extraction Reagent 1, and Extraction Reagent 3 contain sodium azide as a preservative.  Sodium axide can react explosively with copper or lead if allowed to accumulate.  Although the amount of sodium azide in the reagents is minimal, large quatities of water should be used when flushing these reagents down the sink.

Extraction Reagents Reagents 1, 2, and e contain a caustic agent.  In case of skin contact, immediately wash with soap and copious amounts fo water.  In case of eye contact, flush for at least 15 minutes with water.

 

 

 

 

 

Procedure: 

In general, an overnight, gram-positive, beta-hemolytic isolate of streptococcal isolate is required for use in this assay.  Colonies should be chosen from an area demonstrating obvious isolation.  One to four isolated colonies are recomended for grouping testing; however, if colonies are pinpoint, an increased number of colonies, approximately a loopful, should be used.

TEST PROCOTOL:

  1. Allow all reagents to acclimatize to room temperature for 10 minutes prior to use.
  2. Label one 12X75mm test tube for each specimen tested.
  3. Add one drop of Extraction Reagent 1 to each tube.
  4. Using a loop, select 1 to 4 isolated beta-hemolytic colonies and suspend them in Extraction Reagent 1.  If colonies are minute, pick a loopful of colonies, such that the Extraction Reagent 1 becomes turbid.
  5. Add one drop of Extraction Reagent 2 to each tube.
  6. Mix the reaction by tapping the tube with a finger for 10 seconds.
  7. Add five drops of Extraction 3 to each tube.  Mix the reaction as in step 6.
  8. Prior to use, resuspend the Blue Latex Reagent A by inverting the tubes.  Dispense one drop of the latex suspesion onto separate circles on the test card.
  9. Using a pasteur pipette, place one drop of the extract suspension next to the drop of latex suspension in each circle. Ensure that the pipette tip does not touch the latex suspension.
  10. Mix the blue latex and the extract with a wooden applicator stick, using the complete area of the circle.
  11. Gently hand rock the entire card allowing the mixture to flow slowly within the ring area.
  12. At one minute, under normal lighting condidtions, observe for agglutination, or strong clumping, of the blue latex particles.

INTERPRETATION OF RESULTS

Positive Results:

A rapid and significantly stong clumping of the blue latex particles, to form an agglutination pattern indicates an identification of the streptococcal isolate for Lancefield group A.

A weak reaction should be repeated using a heavier inoculum.  The repeated test is considered positive if a visible agglutionation occurs.

Negative Results:

No visible agglutination of the latex particles indicates a negative reaction for Lancefield group A.

Key Points: 

QUALITY CONTROL

Test the kit each day of use with a known positive - a colony from the quality control Group A Strep plate, and with a known negative - a colony from the quality control Group B Strep plate.  Record in the StrepPRO quality control log.

LIMITATIONS

The kit is intended for use in the identification of beta-hemolytic streptococci from a Blood Agar Plate.

TRAVEL CLINIC APPOINTMENTS - STANDARDIZED NURSING PROCEDURES *

Effective Date: 
Mon, 11/01/2010
Reviewed: 
Mon, 08/30/2021
Revised: 
Mon, 08/30/2021

UCSC Student Health Center works with the UCSC Division of Global Engagement (DGE), the UC Education Abroad Program (EAP), and with students traveling abroad outside of campus sponsored programs to prepare students for safe, healthy travel experiences. This procedure delineates the standard elements of an EAP and a non-EAP Travel Consult, both locally coordinated by UCSC DGE. It also provides the process for obtaining UC-wide EAP  and DGE coordinated travel program clearance for participation in one of these programs.

EXPEDITED TREATMENT OF WOMEN WITH URINARY TRACT INFECTIONS - STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Wed, 07/28/2021
Revised: 
Fri, 10/22/2021

This procedure provides specific guidelines to guide the RN in providing expedited treatment of uncomplicated urinary tract infections to women presenting to the UCSC Student Health Center.

Procedure

EAR IRRIGATIONS TO REMOVE IMPACTED CERUMEN - STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Wed, 07/21/2021
Revised: 
Wed, 07/21/2021

This procedure allows an RN to assess the amount of cerumen in the ear canal and to initiate the process of clearing the ear canal when the RN or clinician assesses excess cerumen.

CRYOTHERAPY FOR VERRUCA VULGARIS AND/OR PLANTAR WARTS - STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Sun, 12/01/2002
Reviewed: 
Tue, 11/02/2021
Revised: 
Tue, 11/02/2021

Successful eradication of plantar warts and some common warts frequently requires repetitive treatments. Benign small and usually painless growths, warts are typically raised, rounded, rough and may have significant surrounding callous. This procedure gives the RN authority to use liquid nitrogen to treat plantar warts and common warts after diagnosis and referral from a UCSC clinician. This procedure does not allow treatment of genital or periungal warts.

CONTRACEPTIVE REFILLS - STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Wed, 01/10/2018
Revised: 
Wed, 01/10/2018

This procedure outlines the situations in which the RN may provide a contraceptive refill to patients without a clinician visit.
Procedure
Three Month Contraceptive Recheck

Self-Directed Asymptomatic STI Test Results - Standardized Nursing Procedures

Effective Date: 
Wed, 09/06/2017
Reviewed: 
Wed, 07/21/2021
Revised: 
Wed, 07/21/2021
Policy: 

This Student Health Center policy allows patients to order their own testing for 4 sexually transmitted infections: HIV, Syphillis, Chlamydia and Gonnorhea.  This policy includes permission for patients to order and to self-collect rectal, throat, and/or vaginal swabs for Gonnorhea and/or Chlamydia testing.  The policy reviews the RNs role in reviewing self-directed STI results.

Procedure: 

1. The Student Health Center web page educates students about what tests are available, when to be tested, and what body parts to test.

2. SHOP provides further support to students with additional questions or to those who need assistance in identifying which tests are indicated.

3. Student completes the online ordering questionnaire via their Health e-Messenger account and presents at the lab.

4. Lab provides appropriate supplies and instructions for proper self-collection of urine, throat, vaginal or rectal specimens.

5. Lab draws blood for HIV and/or syphillis testing.

6. RNs review Self-Directed STI lab results every morning.

7. Students with negative test results receive a secure message explaining that all tests from all sites were normal at this time; no actual results are included.  Students are instructed to contact the Medical Records department for actual copies of the test results.  Positive test results are forwarded to the Assoicate Medical Director who contacts the student for appropriate treatment.

Students requesting asymptomatic STI testing are directed to the SHC web site to compete the online questionnaire. Once completed, they present to the lab for self-directed STI testing. Completion of the online questionnarie is required is before testing. It contains information to assist the patient in order the appropriate tests, from the appropriate body parts, after appropriate waiting times for accurate testing. Students requesting further education on STIs and STI risk reduction should be referred to SHOP.

ANAPHYLAXIS EMERGENCY CARE - STANDARDIZED NURSING PROCEDURES *

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Wed, 07/14/2021
Revised: 
Wed, 07/14/2021

This procedure gives the RN the delegated authority to initiate appropriate care of the patient presenting with an anaphylactic reaction. Anaphylactic reactions are rare but life threatening. The timely use of Epinephrine, Benadryl and oxygen is critical to appropriate care of the patient.
Procedure
RN assesses patient and notes evidence of anaphylaxis. Percentages noted in parenthesis indicate likelihood of having this symptom as an indicator of impending anaphylaxis.
Evidence of developing anaphylaxis includes:

ANKLE AND FOOT X-RAY ORDERS - STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Wed, 07/14/2021
Revised: 
Wed, 07/14/2021

This standardized procedure uses the Ottawa Rules for x-ray of the Ankle and the Foot as guidelines to allow the RN to order x-rays prior to clinician appointment when the patient meets the established criteria. In a meta-analysis, the Ottawa Rules for x-ray of the Ankle had a 97.6% sensitivity and reduced the unnecessary use of x-rays by 34%.

Procedure:

b.) GENERIC FORMAT FOR STANDARDIZED NURSING PROCEDURES **

Effective Date: 
Wed, 09/01/2010
Reviewed: 
Fri, 06/04/2021
Revised: 
Thu, 10/06/2016

(developed based on CA Board of Registered Nursing, Title 16, CA Code of Regulations, section 1474)

The introduction to the Specialized Nursing Procedure section of the Nursing Manuel will specify the system by which the procedures are developed and reviewed, the criteria for periodic review, and the standards for continuing education. It will also state that all procedures are done only within the setting of the UCSC Student Health Center.

Procedure
Delineates each step in the procedure
 

Syndicate content