QUEST LABORATORY SPECIMEN COLLECTION PROCEDURES

Effective Date: 
Fri, 07/01/2005
Reviewed: 
Tue, 01/10/2017
Policy: 
SPECIMEN COLLECTION COMMENTS STORAGE FOR TRANSPORT
Blood Aerobic and Anaerobic Bactec bottles, draw aerobic bottle first Room Temperature
Chlamydia Culture 1.  V-C-M medium(green top) tube Refrigerate
Chlamydia Aptima swab Follow package directions.  Cannot be used for any  cultures Refrigerate
Chlamydia/GC (Urine) Sterile  screw top  container, 30  ml  minimum  (at least 1 hr  since
last void)
Refrigerate
Ear/Eye Culture Amies  culturette (blue top) Room Temperature
Fungal Culture Must state source; any culturette; hair, skin etc in clean cup Room Temperature
Genital Culture Amies culturette (blue top) Room Temperature
Giardia Antigen 10%  Formalin container required  (black top) Room temperature
Gram Stain Blue amies swab Room temperature
Herpes Culture 1. V-C-M medium(green top) tube 1. Room Temperature
Nasopharyngeal Culture 1.  Viral = V-C-M medium(green top) tube
2. Bacterial = Wire mini-tip with Amies culturette (green top)
Room Temperature
Ova and Parasites Formalin  (black tops) Room Temperature
Stool Culture 1.  Cary-Blair container required Room Temperature
" " 2.  Amies  culturette (blue top) if QNS  specimen  Room Temperature
" " Indicate if E. coli 0157, Vibrio or Yersenia Room Temperature
Stool for WBCs Formalin container (black top) Room Temperature
Sputum Culture Sterile  screw top  container,  swabs  not acceptable Refrigerate
Ureaplasma/Mycoplasma V-C-M medium(green top) tube Refrigerate
Urine Keep  at  room temp  <  1  hour, must  use  BD Vacutainer  Urine Refrigerate
" " C&S tube (grey top):  located  in  drawer below  Chemstrip  101 Refrigerate
Wound Culture Amies culturette  (blue top);  indicate source.  Clean  surface wounds first to avoid contamination Room Temperature
SPECIMEN COLLECTION  COMMENTS STORAGE  FOR  TRANSPORT
Urine Keep at  room temp <  1  hour REFRIGERATED
  Refrigerate for testing up to 24 hrs REFRIGERATED
     
Stool Culture Cary-Blair container required ROOM TEMP
  Culturette  if QNS specimen ROOM TEMP
  Indicate  if E. coli 0157, Vibrio  or Yersinia ROOM TEMP
     
Ova and Parasites Formalin (black tops) ROOM TEMP
     
Giardia Antigen Formalin container required  (black top) ROOM TEMP
     
Herpes Culture V-C-M medium(green top) tube REFRIGERATED
     
     
Chlamydia Gen-Probe Follow package directions.  Cannot  be
used for any cultures
ROOM TEMP
     
GC Screen Amies culturette  (green top) ROOM TEMP
     
Genital Culture Amies culturette  (blue top) ROOM TEMP
     
Ureaplasma/Mycoplasma V-C-M medium(green top) 4°C
     
Ear/Eye Culture Amies culturette  (blue top) ROOM TEMP
     
Nasopharyngeal Culture Amies culturette (green top) ROOM TEMP
     
Wound Culture Amies culturette  (blue top)  clean surface wounds
first to avoid contamination.
ROOM TEMP
     
Fungal Culture Must state  source;  Any  culturette;  hair,  skin
etc.  in  clean  cup
ROOM TEMP
     
Sputum Culture First  morning  specimen  in  urine cup REFRIGERATED